Barcelona reached the Champions League last eight after beating Lyon 5-1 in the round of 16 second-leg encounter, while Man United defeated Paris Saint-Germain 3-1.
Barca head to the game following a 2-0 victory over Atletico Madrid in the La Liga encounter over the weekend.
Below is Barcelona squad against Man United:
Ter Stegen, N. Semedo, Pique, I. Rakitic, Sergio, Todibo, Coutinho, Arthur, Suarez, Messi, Dembele, Cillessen, Malcom, Lenglet, Murillo, Jordi Alba, Prince, S.Roberto, Vidal, Umtiti, Alena and Inaki Pena.
The kick-off time for the match is 8pm on Wednesday.